• Immunohistochemistry analysis of paraffin-embedded mouse spleen using MEF2A+MEF2C Rabbit monoclonal antibody (STJ11103504) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MEF2A+MEF2C Rabbit monoclonal antibody (STJ11103504) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MEF2A+MEF2C antibody (STJ11103504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MEF2A+MEF2C antibody (250-473) [S4MR] (STJ11103504)

SKU:
STJ11103504

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEF2A
MEF2C
Gene ID: 4205
4208
Uniprot ID: MEF2A_HUMAN
MEF2C_HUMAN
Immunogen Region: 250-473
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 250-473 of human MEF2A+MEF2C (Q06413).
Immunogen Sequence: NRKPDLRVLIPPGSKNTMPS VSEDVDLLLNQRINNSQSAQ SLATPVVSVATPTLPGQGMG GYPSAISTTYGTEYSLSSAD LSSLSGFNTASALHLGSVTG WQQQHLHNMPPSALSQLGAC TSTHLSQSSNLSLPSTQSLN IKSEPVSPPRDRTTTPSRYP QHTRHEAGRSPVDSLSSCSS SYDGSDREDHRNEFHSPIGL TRPSPDERESPSVKRMRLSE GWAT
Tissue Specificity Q02078 - Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
Q06413 - Expressed in brain and skeletal muscle.
Alternative Antibody Names Anti-Myocyte-Specific Enhancer Factor 2a antibody
Anti-Serum Response Factor-Like Protein 1 antibody
Anti-MEF2A antibody
Anti-MEF2 antibody
Anti-Myocyte-Specific Enhancer Factor 2c antibody
Anti-Myocyte Enhancer Factor 2c antibody
Anti-MEF2C antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance