• Immunofluorescence with Anti-MeCP2 isoform 2 Antibody (STJ501735) on wild type and -e1/y male brains. Primary antibody is at 1:100 dilution in buffer, incubated overnight at RT. Secondary incubated for 2 hours at RT.

Anti-MeCP2 isoform 2 antibody (436-486) (STJ501735)

SKU:
STJ501735-100

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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MeCP2 isoform 2 (436-486) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.50 µg/µl
Dilution Range: WB: 1:500
ELISA: 1:10, 000
IP: 1:200
IHC: 1:50-1:100
IF: 1:100
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: MECP2
Gene ID: 4204
Uniprot ID: MECP2_HUMAN
Immunogen Region: 436-486
Specificity: Antibody is specific to MeCP2 e2 and has no cross reactivity with MeCP2 e1.
Immunogen: Synthetic peptide taken within amino acid region 436-486 on Methyl-CpG-binding protein 2 isoform 2 protein.
Tissue Specificity Present in all adult somatic tissues tested.
Post Translational Modifications Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name Methyl-Cpg-Binding Protein 2
Mecp-2 Protein
Mecp2
Database Links Reactome: R-HSA-8986944
Reactome: R-HSA-9022534
Reactome: R-HSA-9022535
Reactome: R-HSA-9022537
Reactome: R-HSA-9022538
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9022707
Reactome: R-HSA-9022927
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Colocalized With Methyl-Cpg In The Genome
Colocalized With Tbl1x To The Heterochromatin Foci
Alternative Antibody Names Anti-Methyl-Cpg-Binding Protein 2 antibody
Anti-Mecp-2 Protein antibody
Anti-Mecp2 antibody
Anti-MECP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance