Host: |
Rabbit |
Applications: |
WB/ICC |
Reactivity: |
Human/Mouse/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-MeCP2 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Purification: |
Antigen Affinity Purified |
Dilution Range: |
WB 1:1000IHCICC 1:100IP |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
Mecp2 |
Gene ID: |
17257 |
Uniprot ID: |
MECP2_MOUSE |
Immunogen Region: |
C-Term |
Specificity: |
The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells, rat PC12 cells, and mouse brain tissue. |
Immunogen: |
MeCP2 synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. |
Post Translational Modifications | Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. |
Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
Database Links | Reactome: R-MMU-9022692 Q9Z2D6-1 |
Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-Mecp2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance