Anti-MECP2 antibody (313-363 aa) (STJ197348)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide derived from MeCP2 at the amino acid range 313-363
General Information
| Short Description | Rabbit polyclonal anti-Methyl-CpG-binding protein 2 (313-363 aa) for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:2000 |
| Formulation | Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MECP2 |
| Gene ID | 4204 |
| Uniprot ID | MECP2_HUMAN |
| Immunogen | Synthetic peptide derived from MeCP2 at the amino acid range 313-363 |
| Immunogen Region | 313-363 aa |
| Specificity | The antibody detects endogenous MeCP2 proteins. |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-HSA-8986944Reactome: R-HSA-9022534Reactome: R-HSA-9022535Reactome: R-HSA-9022537Reactome: R-HSA-9022538Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9022702Reactome: R-HSA-9022707Reactome: R-HSA-9022927Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-MECP2 antibody |
Information sourced from Uniprot.org