• Western blot analysis of extracts of various cell lines, using ME2 antibody (STJ111777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using ME2 antibody (STJ111777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug ME2 antibody (STJ111777). Western blot was performed from the immunoprecipitate using ME2 antibody (STJ111777) at a dilition of 1:1000.

Anti-ME2 antibody (220-479) (STJ111777)

SKU:
STJ111777

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ME2 (220-479) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ME2
Gene ID: 4200
Uniprot ID: MAOM_HUMAN
Immunogen Region: 220-479
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-479 of human ME2 (NP_001161807.1).
Immunogen Sequence: GLYQKRDRTQQYDDLIDEFM KAITDRYGRNTLIQFEDFGN HNAFRFLRKYREKYCTFNDD IQGTAAVALAGLLAAQKVIS KPISEHKILFLGAGEAALGI ANLIVMSMVENGLSEQEAQK KIWMFDKYGLLVKGRKAKID SYQEPFTHSAPESIPDTFED AVNILKPSTIIGVAGAGRLF TPDVIRAMASINERPVIFAL SNPTAQAECTAEEAYTLTEG RCLFASGSPFGPVKLTDGR
Function NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.
Protein Name Nad-Dependent Malic Enzyme - Mitochondrial
Nad-Me
Malic Enzyme 2
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nad-Dependent Malic Enzyme - Mitochondrial antibody
Anti-Nad-Me antibody
Anti-Malic Enzyme 2 antibody
Anti-ME2 antibody

Information sourced from Uniprot.org

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