• Western blot analysis of extracts of various cell lines, using ME1 antibody (STJ24535) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ME1 antibody (1-300) (STJ24535)

SKU:
STJ24535

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ME1
Gene ID: 4199
Uniprot ID: MAOX_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1).
Immunogen Sequence: MEPEAPRRRHTHQRGYLLTR NPHLNKDLAFTLEERQQLNI HGLLPPSFNSQEIQVLRVVK NFEHLNSDFDRYLLLMDLQD RNEKLFYRVLTSDIEKFMPI VYTPTVGLACQQYSLVFRKP RGLFITIHDRGHIASVLNAW PEDVIKAIVVTDGERILGLG DLGCNGMGIPVGKLALYTAC GGMNPQECLPVILDVGTENE ELLKDPLYIGLRQRRVRGSE YDDFLDEFMEAVSSKYGMN
Tissue Specificity Expressed in all tissues tested including liver, placenta and white adipose tissue.
Function Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.
Protein Name Nadp-Dependent Malic Enzyme
Nadp-Me
Malic Enzyme 1
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-70268
Reactome: R-HSA-9818025
Reactome: R-HSA-9861718
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nadp-Dependent Malic Enzyme antibody
Anti-Nadp-Me antibody
Anti-Malic Enzyme 1 antibody
Anti-ME1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance