Western blot analysis of various lysates using MDH1 Rabbit mAb (STJ11102525) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-MDH1 antibody (235-334) [S5MR] (STJ11102525)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102525
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General Information

Short DescriptionRabbit monoclonal MDH1 (235-334) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMDH1
Gene ID4190
Uniprot IDMDHC_HUMAN
Immunogen Region235-334
Immunogen SequenceIKARKLSSAMSAAKAICDHV RDIWFGTPEGEFVSMGVISD GNSYGVPDDLLYSFPVVIKN KTWKFVEGLPINDFSREKMD LTAKELTEEKESAFEFLSSA
SpecificityA synthetic peptide corresponding to a sequence within amino acids 235-334 of human MDH1 (P40925).

Additional Info

Post Translational Modifications ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
Function Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).
Protein Name Malate Dehydrogenase - Cytoplasmic
Aromatic Alpha-Keto Acid Reductase
Kar
Cytosolic Malate Dehydrogenase
Database Links Reactome: R-HSA-9856872
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Malate Dehydrogenase - Cytoplasmic antibody
Anti-Aromatic Alpha-Keto Acid Reductase antibody
Anti-Kar antibody
Anti-Cytosolic Malate Dehydrogenase antibody
Anti-MDH1 antibody
Anti-MDHA antibody

Information sourced from Uniprot.org

Citations

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