Anti-MDH1 antibody (1-334) (STJ29701)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MDH1 (1-334) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MDH1 |
| Gene ID | 4190 |
| Uniprot ID | MDHC_HUMAN |
| Immunogen Region | 1-334 |
| Immunogen Sequence | MSEPIRVLVTGAAGQIAYSL LYSIGNGSVFGKDQPIILVL LDITPMMGVLDGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSMPRREGMERKD LLKANVKIFKSQGAALDKYA KKSVKVIVVGNPANTNCLTA SKSAPSIPKENFSCLTRLDH NRAKAQIALKLGVTANDVKN VIIWGNHSSTQYPDVNHAKV KLQGKEVGVYEALKDDSWLK GEFVTTVQQRGAAVIKARK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1). |
Additional Info
| Post Translational Modifications | ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation. |
| Function | Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS). |
| Protein Name | Malate Dehydrogenase - CytoplasmicAromatic Alpha-Keto Acid ReductaseKarCytosolic Malate Dehydrogenase |
| Database Links | Reactome: R-HSA-9856872 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Malate Dehydrogenase - Cytoplasmic antibodyAnti-Aromatic Alpha-Keto Acid Reductase antibodyAnti-Kar antibodyAnti-Cytosolic Malate Dehydrogenase antibodyAnti-MDH1 antibodyAnti-MDHA antibody |
Information sourced from Uniprot.org