• Western blot analysis of extracts of various cell lines, using MDH1 antibody (STJ29701) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MDH1 antibody (1-334) (STJ29701)

SKU:
STJ29701

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDH1
Gene ID: 4190
Uniprot ID: MDHC_HUMAN
Immunogen Region: 1-334
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1).
Immunogen Sequence: MSEPIRVLVTGAAGQIAYSL LYSIGNGSVFGKDQPIILVL LDITPMMGVLDGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSMPRREGMERKD LLKANVKIFKSQGAALDKYA KKSVKVIVVGNPANTNCLTA SKSAPSIPKENFSCLTRLDH NRAKAQIALKLGVTANDVKN VIIWGNHSSTQYPDVNHAKV KLQGKEVGVYEALKDDSWLK GEFVTTVQQRGAAVIKARK
Post Translational Modifications ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
Function Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).
Protein Name Malate Dehydrogenase - Cytoplasmic
Aromatic Alpha-Keto Acid Reductase
Kar
Cytosolic Malate Dehydrogenase
Database Links Reactome: R-HSA-9856872
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Malate Dehydrogenase - Cytoplasmic antibody
Anti-Aromatic Alpha-Keto Acid Reductase antibody
Anti-Kar antibody
Anti-Cytosolic Malate Dehydrogenase antibody
Anti-MDH1 antibody
Anti-MDHA antibody

Information sourced from Uniprot.org

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