![Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176827/367792/STJ11104182_1__60361.1713133337.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176827/367793/STJ11104182_2__88718.1713133337.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/176827/367792/STJ11104182_1__60361.1713133337.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
Anti-MCM6 antibody (601-821) [S4182RM] (STJ11104182)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Dna Replication Licensing Factor Mcm6 (601-821) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4182RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCM6 |
| Gene ID | 4175 |
| Uniprot ID | MCM6_HUMAN |
| Immunogen Region | 601-821 |
| Immunogen Sequence | LRQRDGSGVTKSSWRITVRQ LESMIRLSEAMARMHCCDEV QPKHVKEAFRLLNKSIIRVE TPDVNLDQEEEIQMEVDEGA GGINGHADSPAPVNGINGYN EDINQESAPKASLRLGFSEY CRISNLIVLHLRKVEEEEDE SALKRSELVNWYLKEIESEI DSEEELINKKRIIEKVIHRL THYDHVLIELTQAGLKGSTE GSESYEEDPYLVVNPNYLLE D |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 601-821 of human MCM6 (NP_005906.2). |
Additional Info
| Post Translational Modifications | O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
| Protein Name | Dna Replication Licensing Factor Mcm6P105mcm |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | NucleusChromosomeBinds To Chromatin During G1 And Detaches From It During S Phase |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm6 antibodyAnti-P105mcm antibodyAnti-MCM6 antibody |
Information sourced from Uniprot.org