• Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g MCM6 antibody (STJ24526). Western blot was performed from the immunoprecipitate using MCM6 antibody (STJ24526) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using MCM6 Rabbit polyclonal antibody (STJ24526). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Western blot analysis of various lysates using MCM6 Rabbit polyclonal antibody (STJ24526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MCM6 antibody (562-821) (STJ24526)

SKU:
STJ24526

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCM6 (562-821) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM6
Gene ID: 4175
Uniprot ID: MCM6_HUMAN
Immunogen Region: 562-821
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).
Immunogen Sequence: SIDRVYSLDDIRRYLLFARQ FKPKISKESEDFIVEQYKHL RQRDGSGVTKSSWRITVRQL ESMIRLSEAMARMHCCDEVQ PKHVKEAFRLLNKSIIRVET PDVNLDQEEEIQMEVDEGAG GINGHADSPAPVNGINGYNE DINQESAPKASLRLGFSEYC RISNLIVLHLRKVEEEEDES ALKRSELVNWYLKEIESEID SEEELINKKRIIEKVIHRLT HYDHVLIELTQAGLKGSTE
Post Translational Modifications O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Protein Name Dna Replication Licensing Factor Mcm6
P105mcm
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-176974
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Nucleus
Chromosome
Binds To Chromatin During G1 And Detaches From It During S Phase
Alternative Antibody Names Anti-Dna Replication Licensing Factor Mcm6 antibody
Anti-P105mcm antibody
Anti-MCM6 antibody

Information sourced from Uniprot.org

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