| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MCM2 |
| Gene ID: | 4171 |
| Uniprot ID: | MCM2_HUMAN |
| Immunogen Region: | 360-700 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MCM2 (NP_004517.2). |
| Immunogen Sequence: | PFEVNMEETIYQNYQRIRIQ ESPGKVAAGRLPRSKDAILL ADLVDSCKPGDEIELTGIYH NNYDGSLNTANGFPVFATVI LANHVAKKDNKVAVGELTDE DVKMITSLSKDQQIGEKIFA SIAPSIYGHEDIKRGLALAL FGGEPKNPGGKHKVRGDINV LLCGDPGTAKSQFLKYIEKV SSRAIFTTGQGASAVGLTAY VQRHPVSREWTLEAGALVLA DRGVCLIDEFDKMNDQDRT |
| Post Translational Modifications | Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis. |
| Protein Name | Dna Replication Licensing Factor Mcm2 Minichromosome Maintenance Protein 2 Homolog Nuclear Protein Bm28 |
| Database Links | Reactome: R-HSA-176187 Reactome: R-HSA-176974 Reactome: R-HSA-68867 Reactome: R-HSA-68949 Reactome: R-HSA-68962 Reactome: R-HSA-69052 |
| Cellular Localisation | Nucleus Chromosome Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm2 antibody Anti-Minichromosome Maintenance Protein 2 Homolog antibody Anti-Nuclear Protein Bm28 antibody Anti-MCM2 antibody Anti-BM28 antibody Anti-CCNL1 antibody Anti-CDCL1 antibody Anti-KIAA0030 antibody |
Information sourced from Uniprot.org