Anti-MCL1 antibody [R04-3A4] (STJA0033895)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human MCL1
General Information
| Short Description | Rabbit monoclonal anti-MCL1 for use in WB, ICC, IF and IP in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-3A4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MCL1 |
| Gene ID | 4170 |
| Uniprot ID | MCL1_HUMAN |
| Immunogen | A synthetic peptide of human MCL1 |
Additional Info
| Post Translational Modifications | Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. Deubiquitinated by USP20.leading to increased stability. |
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Protein Name | Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1Bcl-2-Like Protein 3Bcl2-L-3Bcl-2-Related Protein Eat/Mcl1Mcl1/Eat |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9725370 |
| Cellular Localisation | MembraneSingle-Pass Membrane ProteinCytoplasmMitochondrionNucleusNucleoplasmCytoplasmicAssociated With Mitochondria |
| Alternative Antibody Names | Anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 antibodyAnti-Bcl-2-Like Protein 3 antibodyAnti-Bcl2-L-3 antibodyAnti-Bcl-2-Related Protein Eat/Mcl1 antibodyAnti-Mcl1/Eat antibodyAnti-MCL1 antibodyAnti-BCL2L3 antibody |
Information sourced from Uniprot.org