Anti-MCL1 antibody (150-250) (STJ24520)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MCL1 (150-250) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCL1 |
| Gene ID | 4170 |
| Uniprot ID | MCL1_HUMAN |
| Immunogen Region | 150-250 |
| Immunogen Sequence | SGNNTSTDGSLPSTPPPAEE EEDELYRQSLEIISRYLREQ ATGAKDTKPMGRSGATSRKA LETLRRVGDGVQRNHETAFQ GMLRKLDIKNEDDVKSLSRV M |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human MCL1 (NP_068779.1). |
Additional Info
| Post Translational Modifications | Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. Deubiquitinated by USP20.leading to increased stability. |
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Protein Name | Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1Bcl-2-Like Protein 3Bcl2-L-3Bcl-2-Related Protein Eat/Mcl1Mcl1/Eat |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9725370 |
| Cellular Localisation | MembraneSingle-Pass Membrane ProteinCytoplasmMitochondrionNucleusNucleoplasmCytoplasmicAssociated With Mitochondria |
| Alternative Antibody Names | Anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 antibodyAnti-Bcl-2-Like Protein 3 antibodyAnti-Bcl2-L-3 antibodyAnti-Bcl-2-Related Protein Eat/Mcl1 antibodyAnti-Mcl1/Eat antibodyAnti-MCL1 antibodyAnti-BCL2L3 antibody |
Information sourced from Uniprot.org