• STJ71781 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MBNL1 antibody (C-Term) (STJ71781)

SKU:
STJ71781-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MBNL1 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MBNL1
Gene ID: 4154
Uniprot ID: MBNL1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_066368.2; NP_997175.1; NP_997176.1; NP_997177.1; NP_997178.1; NP_997179.1; NP_997180.1
Specificity: This antibody is expected to recognize the reported isoforms (NP_066368.2; NP_997175.1; NP_997176.1; NP_997177.1; NP_997178.1; NP_997179.1)
Immunogen Sequence: SAEHLTSHKYVTQ
Function Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Protein Name Muscleblind-Like Protein 1
Triplet-Expansion Rna-Binding Protein
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Granule
Localized With Ddx1
Tial1 And Ybx1 In Stress Granules Upon Stress
Localized In The Cytoplasm Of Multinucleated Myotubes
Colocalizes With Nuclear Foci Of Retained Expanded-Repeat Transcripts In Myotubes From Patients Affected By Myotonic Dystrophy
Alternative Antibody Names Anti-Muscleblind-Like Protein 1 antibody
Anti-Triplet-Expansion Rna-Binding Protein antibody
Anti-MBNL1 antibody
Anti-EXP antibody
Anti-KIAA0428 antibody
Anti-MBNL antibody

Information sourced from Uniprot.org

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