Anti-MBD5 antibody (1057-1107 aa) (STJ197935)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human MBD5 at the amino acid range 1057-1107
General Information
| Short Description | Rabbit polyclonal anti-Methyl-CpG-binding domain protein 5 (1057-1107 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MBD5 |
| Gene ID | 55777 |
| Uniprot ID | MBD5_HUMAN |
| Immunogen | Synthesized peptide derived from the human MBD5 at the amino acid range 1057-1107 |
| Immunogen Region | 1057-1107 aa |
| Specificity | This antibody detects endogenous levels of human MBD5 |
Additional Info
| Function | Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1). Important for stability of PR-DUB components and stimulating its ubiquitinase activity. As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3. The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in cell growth and survivability. MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways. Heterochromatin recruitment is not mediated by DNA methylation. The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 5Methyl-Cpg-Binding Protein Mbd5 |
| Database Links | Reactome: R-HSA-5689603 |
| Cellular Localisation | Isoform 1: NucleusChromosomeAssociates With Heterochromatin Containing Chromocentres In A Dna Methylation-Independent MannerDoes Not Localize To Sites Of Dna DamageIsoform 2: NucleusDoes Not Associate With Heterochromatin Containing Chromocentres |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Domain Protein 5 antibodyAnti-Methyl-Cpg-Binding Protein Mbd5 antibodyAnti-MBD5 antibodyAnti-KIAA1461 antibody |
Information sourced from Uniprot.org