Anti-MBD1 antibody [R02-7E9] (STJA0036479)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human MBD1
General Information
| Short Description | Rabbit monoclonal anti-MBD1 for use in WB, IHC-P, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-7E9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MBD1 |
| Gene ID | 4152 |
| Uniprot ID | MBD1_HUMAN |
| Immunogen | A synthesized peptide derived from human MBD1 |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Sumoylated, sumoylation may increase interaction with ATF7IP. |
| Function | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 1Cxxc-Type Zinc Finger Protein 3Methyl-Cpg-Binding Protein Mbd1Protein Containing Methyl-Cpg-Binding Domain 1 |
| Database Links | Reactome: R-HSA-3899300 |
| Cellular Localisation | NucleusNucleus MatrixNucleus SpeckleChromosomeNuclearIn A Punctate PatternAssociated With Euchromatic Regions Of The ChromosomesWith Pericentromeric Regions On Chromosome 1 And With Telomeric Regions From Several Chromosomes |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Domain Protein 1 antibodyAnti-Cxxc-Type Zinc Finger Protein 3 antibodyAnti-Methyl-Cpg-Binding Protein Mbd1 antibodyAnti-Protein Containing Methyl-Cpg-Binding Domain 1 antibodyAnti-MBD1 antibodyAnti-CXXC3 antibodyAnti-PCM1 antibody |
Information sourced from Uniprot.org