• Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Myoglobin Rabbit polyclonal antibody (STJ27424) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using Myoglobin Rabbit polyclonal antibody (STJ27424) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Myoglobin Rabbit polyclonal antibody (STJ27424) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : Rat liver. Exposuretime: 1s.

Anti-MB antibody (1-154) (STJ27424)

SKU:
STJ27424

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MB
Gene ID: 4151
Uniprot ID: MYG_HUMAN
Immunogen Region: 1-154
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human Myoglobin (NP_005359.1).
Immunogen Sequence: MGLSDGEWQLVLNVWGKVEA DIPGHGQEVLIRLFKGHPET LEKFDKFKHLKSEDEMKASE DLKKHGATVLTALGGILKKK GHHEAEIKPLAQSHATKHKI PVKYLEFISECIIQVLQSKH PGDFGADAQGAMNKALELFR KDMASNYKELGFQG
Function Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity.
Protein Name Myoglobin
Nitrite Reductase Mb
Pseudoperoxidase Mb
Database Links Reactome: R-HSA-8981607
Cellular Localisation Cytoplasm
Sarcoplasm
Alternative Antibody Names Anti-Myoglobin antibody
Anti-Nitrite Reductase Mb antibody
Anti-Pseudoperoxidase Mb antibody
Anti-MB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance