Anti-Mast Cell Tryptase antibody (30-275) [S4MR] (STJ11103144)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Mast Cell Tryptase (TPSB2) (30-275) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:100-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TPSAB1 |
| Gene ID | 7177 |
| Uniprot ID | TRYB1_HUMAN |
| Immunogen Region | 30-275 |
| Immunogen Sequence | GIVGGQEAPRSKWPWQVSLR VHGPYWMHFCGGSLIHPQWV LTAAHCVGPDVKDLAALRVQ LREQHLYYQDQLLPVSRIIV HPQFYTAQIGADIALLELEE PVNVSSHVHTVTLPPASETF PPGMPCWVTGWGDVDNDERL PPPFPLKQVKVPIMENHICD AKYHLGAYTGDDVRIVRDDM LCAGNTRRDSCQGDSGGPLV CKVNGTWLQAGVVSWGEGCA QPNRPGIYTRVTYYLDWIH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-275 of human Mast Cell Tryptase (TPSB2) (TPSB2) (P20231). |
Additional Info
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin.in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level). |
| Function | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. |
| Protein Name | Tryptase Alpha/Beta-1Tryptase-1Tryptase ITryptase Alpha-1 |
| Database Links | Reactome: R-HSA-1592389 |
| Cellular Localisation | SecretedReleased From The Secretory Granules Upon Mast Cell Activation |
| Alternative Antibody Names | Anti-Tryptase Alpha/Beta-1 antibodyAnti-Tryptase-1 antibodyAnti-Tryptase I antibodyAnti-Tryptase Alpha-1 antibodyAnti-TPSAB1 antibodyAnti-TPS1 antibodyAnti-TPS2 antibodyAnti-TPSB1 antibody |
Information sourced from Uniprot.org