• STJ71509 (0. 3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MARK2/PAR-1 antibody (Internal) (STJ71509)

SKU:
STJ71509-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MARK2/PAR-1 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MARK2
Gene ID: 2011
Uniprot ID: MARK2_HUMAN
Immunogen Region: Internal
Accession Number: NP_004945.4; NP_001156768.1; NP_001156769.1
Specificity: This antibody is expected to recognise isoform c (NP_004945.4) , isoform e (NP_001156768.1) and isoform f (NP_001156769.1).
Immunogen Sequence: QNGKDSTAPQR
Post Translational Modifications Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm.
Function Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Protein Name Serine/Threonine-Protein Kinase Mark2
Elkl Motif Kinase 1
Emk-1
Map/Microtubule Affinity-Regulating Kinase 2
Par1 Homolog
Par1 Homolog B
Par-1b
Par1b
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasm
Lateral Cell Membrane
Cytoskeleton
Cell Projection
Dendrite
Phosphorylation At Thr-596 By Prkcz/Apkc And Subsequent Interaction With 14-3-3 Protein Ywhaz Promotes Relocation From The Cell Membrane To The Cytoplasm
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Mark2 antibody
Anti-Elkl Motif Kinase 1 antibody
Anti-Emk-1 antibody
Anti-Map/Microtubule Affinity-Regulating Kinase 2 antibody
Anti-Par1 Homolog antibody
Anti-Par1 Homolog B antibody
Anti-Par-1b antibody
Anti-Par1b antibody
Anti-MARK2 antibody
Anti-EMK1 antibody

Information sourced from Uniprot.org

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