Anti-MARCKSL1 antibody (1-195) (STJ29212)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ29212
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General Information

Short DescriptionRabbit polyclonal MARCKSL1 (1-195) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMARCKSL1
Gene ID65108
Uniprot IDMRP_HUMAN
Immunogen Region1-195
Immunogen SequenceMGSQSSKAPRGDVTAEEAAG ASPAKANGQENGHVKSNGDL SPKGEGESPPVNGTDEAAGA TGDAIEPAPPSQGAEAKGEV PPKETPKKKKKFSFKKPFKL SGLSFKRNRKEGGGDSSASS PTEEEQEQGEIGACSDEGTA QEGKAAATPESQEPQAKGAE ASAASEEEAGPQATEPSTPS GPESGPTPASAEQNE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MARCKSL1 (NP_075385.1).

Additional Info

Post Translational Modifications Phosphorylated. Phosphorylation at Ser-120 and Thr-178 is non-redundantly catalyzed by MAPK8 in vivo. Phosphorylation at Thr-148 is preferentially catalyzed by MAPK8 in vivo, but this modification can also be catalyzed by other kinases in the absence of MAPK8. May be phosphorylated by protein kinase C, which disrupts the interaction with calmodulin.
Function Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems.
Protein Name Marcks-Related Protein
Marcks-Like Protein 1
Macrophage Myristoylated Alanine-Rich C Kinase Substrate
Mac-Marcks
Macmarcks
Database Links
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Lipid-Anchor
Associates With The Membrane Via The Insertion Of The N-Terminal N-Myristoyl Chain And The Partial Insertion Of The Effector Domain
Association Of The Effector Domain With Membranes May Be Regulated By Ca(2+)/Calmodulin
Colocalizes With F-Actin At The Leading Edge Of Migrating Cells
In Prostate Cancers
Shows Strong Expression At Apical And/Or Basal Regions Of The Cell And Also Has Weak Cytoplasmic Expression
Alternative Antibody Names Anti-Marcks-Related Protein antibody
Anti-Marcks-Like Protein 1 antibody
Anti-Macrophage Myristoylated Alanine-Rich C Kinase Substrate antibody
Anti-Mac-Marcks antibody
Anti-Macmarcks antibody
Anti-MARCKSL1 antibody
Anti-MLP antibody
Anti-MRP antibody

Information sourced from Uniprot.org

Citations

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