Anti-MAPT antibody (3-214 aa) (STJ11105180)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MAPT (3-214 aa) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Mapt |
| Gene ID | 17762 |
| Uniprot ID | TAU_MOUSE |
| Immunogen Region | 3-214 aa |
| Immunogen Sequence | DPRQEFDTMEDHAGDYTLLQ DQEGDMDHGLKAEEAGIGDT PNQEDQAAGHVTQARVASKD RTGNDEKKAKGADGKTGAKI ATPRGAASPAQKGTSNATRI PAKTTPSPKTPPGSGEPPKS GERSGYSSPGSPGTPGSRSR TPSLPTPPTREPKKVAVVRT PPKSPSASKSRLQTAPVPMP DLKNVRSKIGSTENLKHQPG GGKVQIINKKLD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 3-214 of mouse MAPT. (NP_034968.3). |
Additional Info
| Tissue Specificity | Expressed in neurons and at a lower level in the liver and kidney. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. |
| Post Translational Modifications | Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome. Phosphorylation at various serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1, CDK1, CDK5, GSK3, MAPK) (a few sites per protein in interphase, more in mitosis), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1, MARK2, MARK3, MARK4), causing detachment from microtubules, and their disassembly. Phosphorylated by PHK. Dephosphorylation at several serine and threonine residues by the serine/threonine phosphatase PPP5C. Phosphorylation at Ser-554 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylation at Ser-188 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. |
| Function | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. |
| Protein Name | Microtubule-Associated Protein TauNeurofibrillary Tangle ProteinPaired Helical Filament-TauPhf-Tau |
| Database Links | Reactome: R-MMU-264870Reactome: -MMU-9833482 |
| Cellular Localisation | CytoplasmCytosolCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoskeletonCell ProjectionAxonDendriteSecretedMostly Found In The Axons Of NeuronsIn The Cytosol And In Association With Plasma Membrane ComponentsCan Be SecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein Tau antibodyAnti-Neurofibrillary Tangle Protein antibodyAnti-Paired Helical Filament-Tau antibodyAnti-Phf-Tau antibodyAnti-Mapt antibodyAnti-Mtapt antibodyAnti-Tau antibody |
Information sourced from Uniprot.org