• Western blot analysis of various lysates using MAPK12 Rabbit polyclonal antibody (STJ115013) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAPK12 antibody (248-367) (STJ115013)

SKU:
STJ115013

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAPK12 (248-367) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK12
Gene ID: 6300
Uniprot ID: MK12_HUMAN
Immunogen Region: 248-367
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 248-367 of human MAPK12 (NP_002960.2).
Immunogen Sequence: EFVQRLQSDEAKNYMKGLPE LEKKDFASILTNASPLAVNL LEKMLVLDAEQRVTAGEALA HPYFESLHDTEDEPQVQKYD DSFDDVDRTLDEWKRVTYKE VLSFKPPRQLGARVSKETPL
Tissue Specificity Highly expressed in skeletal muscle and heart.
Post Translational Modifications Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme. Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes.and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
Protein Name Mitogen-Activated Protein Kinase 12
Map Kinase 12
Mapk 12
Extracellular Signal-Regulated Kinase 6
Erk-6
Mitogen-Activated Protein Kinase P38 Gamma
Map Kinase P38 Gamma
Stress-Activated Protein Kinase 3
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-171007
Reactome: R-HSA-2151209
Reactome: R-HSA-376172
Reactome: R-HSA-4420097
Reactome: R-HSA-525793
Reactome: R-HSA-5675221
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Mitochondrial When Associated With Sh3bp5
In Skeletal Muscle Colocalizes With Snta1 At The Neuromuscular Junction And Throughout The Sarcolemma
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 12 antibody
Anti-Map Kinase 12 antibody
Anti-Mapk 12 antibody
Anti-Extracellular Signal-Regulated Kinase 6 antibody
Anti-Erk-6 antibody
Anti-Mitogen-Activated Protein Kinase P38 Gamma antibody
Anti-Map Kinase P38 Gamma antibody
Anti-Stress-Activated Protein Kinase 3 antibody
Anti-MAPK12 antibody
Anti-ERK6 antibody
Anti-SAPK3 antibody

Information sourced from Uniprot.org

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