Anti-MAP3K14 antibody (250-350) (STJ501669)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 250-350 on human MAP3K14 protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-MAP3K14 (250-350) is suitable for use in ELISA and Western Blot research applications. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.64-0.72 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP3K14 |
| Gene ID | 9020 |
| Uniprot ID | M3K14_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 250-350 on human MAP3K14 protein. |
| Immunogen Region | 250-350 |
Additional Info
| Tissue Specificity | Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. |
| Post Translational Modifications | Autophosphorylated. Phosphorylation at Thr-559 is required to activate its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
| Function | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway. Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response. Could act in a receptor-selective manner. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 14Nf-Kappa-Beta-Inducing KinaseHsnikSerine/Threonine-Protein Kinase Nik |
| Database Links | Reactome: R-HSA-389357Reactome: R-HSA-5607761Reactome: R-HSA-5668541Reactome: R-HSA-5676590Reactome: R-HSA-5676594 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 14 antibodyAnti-Nf-Kappa-Beta-Inducing Kinase antibodyAnti-Hsnik antibodyAnti-Serine/Threonine-Protein Kinase Nik antibodyAnti-MAP3K14 antibodyAnti-NIK antibody |
Information sourced from Uniprot.org