Western blot analysis of various lysates using MAP3K1 Rabbit pAb (STJ118497) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MAP3K1 antibody (1400-1512) (STJ118497)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ118497
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General Information

Short DescriptionRabbit polyclonal MAP3K1 (1400-1512) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:4000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAP3K1
Gene ID4214
Uniprot IDM3K1_HUMAN
Immunogen Region1400-1512
Immunogen SequenceTGAGEFQGQLLGTIAFMAPE VLRGQQYGRSCDVWSVGCAI IEMACAKPPWNAEKHSNHLA LIFKIASATTAPSIPSHLSP GLRDVALRCLELQPQDRPPS RELLKHPVFRTTW
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1400-1512 of human MAP3K1 (NP_005912.1).

Additional Info

Post Translational Modifications Autophosphorylated.
Function Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 1
Mapk/Erk Kinase Kinase 1
Mek Kinase 1
Mekk 1
Database Links Reactome: R-HSA-166058
Reactome: R-HSA-2871796
Reactome: R-HSA-933542
Reactome: R-HSA-975138
Reactome: R-HSA-975871
Cellular Localisation
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 antibody
Anti-Mapk/Erk Kinase Kinase 1 antibody
Anti-Mek Kinase 1 antibody
Anti-Mekk 1 antibody
Anti-MAP3K1 antibody
Anti-MAPKKK1 antibody
Anti-MEKK antibody
Anti-MEKK1 antibody

Information sourced from Uniprot.org

Citations

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