| Host: | Mouse |
| Applications: | WB/IF/FC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Dual specificity mitogen-activated protein kinase kinase 2 is suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 7F5 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP2K2 |
| Gene ID: | 5605 |
| Uniprot ID: | MP2K2_HUMAN |
| Specificity: | MEK-2 Monoclonal Antibody detects endogenous levels of MEK-2 protein. |
| Immunogen: | Purified recombinant fragment of human MEK-2 expressed in E. Coli. |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 2Map Kinase Kinase 2Mapkk 2Erk Activator Kinase 2Mapk/Erk Kinase 2Mek 2 |
| Database Links | Reactome: R-HSA-112411Reactome: R-HSA-170968Reactome: R-HSA-445144Reactome: R-HSA-5210891Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinMembrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibodyAnti-Map Kinase Kinase 2 antibodyAnti-Mapkk 2 antibodyAnti-Erk Activator Kinase 2 antibodyAnti-Mapk/Erk Kinase 2 antibodyAnti-Mek 2 antibodyAnti-MAP2K2 antibodyAnti-MEK2 antibodyAnti-MKK2 antibodyAnti-PRKMK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90529/360110/STJ11101615_1__89635.1713124721.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
