• Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A Mouse mAb diluted at 1:200.
  • Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A Mouse mAb diluted at 1:1000.
  • Immunofluorescence analysis of Mouse-spleen tissue. 1, LC3A Mouse monoclonal antibody (5G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A Mouse mAb diluted at 1:200.
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, LC3A Mouse monoclonal antibody (5G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, LC3A Mouse monoclonal antibody (5G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, LC3A Mouse monoclonal antibody (5G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.
  • Immunofluorescence analysis of Hela cell. 1, CREB-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MAP1LC3A antibody [5G10] (STJ97755)

SKU:
STJ97755

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Microtubule-associated proteins 1A/1B light chain 3A is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 5G10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
IHC 1:100-200
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP1LC3A
Gene ID: 84557
Uniprot ID: MLP3A_HUMAN
Specificity: LC3A protein detects endogenous levels of LC3A
Immunogen: Synthetic Peptide of LC3A
Post Translational Modifications The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE).
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
Protein Name Microtubule-Associated Proteins 1a/1b Light Chain 3a
Autophagy-Related Protein Lc3 A
Autophagy-Related Ubiquitin-Like Modifier Lc3 A
Map1 Light Chain 3-Like Protein 1
Map1a/Map1b Light Chain 3 A
Map1a/Map1b Lc3 A
Microtubule-Associated Protein 1 Light Chain 3 Alpha
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8934903
Cellular Localisation Cytoplasmic Vesicle
Autophagosome Membrane
Lipid-Anchor
Endomembrane System
Cytoplasm
Cytoskeleton
Lc3-Ii Binds To The Autophagic Membranes
Alternative Antibody Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody

Information sourced from Uniprot.org

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