| Host: | Mouse |
| Applications: | WB/IF/IHC |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Microtubule-associated proteins 1A/1B light chain 3A is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5G10 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 IHC 1:100-200 IF 1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP1LC3A |
| Gene ID: | 84557 |
| Uniprot ID: | MLP3A_HUMAN |
| Specificity: | LC3A protein detects endogenous levels of LC3A |
| Immunogen: | Synthetic Peptide of LC3A |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). |
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. |
| Protein Name | Microtubule-Associated Proteins 1a/1b Light Chain 3a Autophagy-Related Protein Lc3 A Autophagy-Related Ubiquitin-Like Modifier Lc3 A Map1 Light Chain 3-Like Protein 1 Map1a/Map1b Light Chain 3 A Map1a/Map1b Lc3 A Microtubule-Associated Protein 1 Light Chain 3 Alpha |
| Database Links | Reactome: R-HSA-1632852 Reactome: R-HSA-5205685 Reactome: R-HSA-8934903 |
| Cellular Localisation | Cytoplasmic Vesicle Autophagosome Membrane Lipid-Anchor Endomembrane System Cytoplasm Cytoskeleton Lc3-Ii Binds To The Autophagic Membranes |
| Alternative Antibody Names | Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody Anti-Autophagy-Related Protein Lc3 A antibody Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody Anti-Map1 Light Chain 3-Like Protein 1 antibody Anti-Map1a/Map1b Light Chain 3 A antibody Anti-Map1a/Map1b Lc3 A antibody Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody Anti-MAP1LC3A antibody |
Information sourced from Uniprot.org