• Western blot analysis of extracts of HeLa cells, using Monoamine Oxidase B (Monoamine Oxidase B (MAOB) ) antibody (STJ24454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using Monoamine Oxidase B (Monoamine Oxidase B (MAOB) ) rabbit polyclonal antibody (STJ24454) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Monoamine Oxidase B (Monoamine Oxidase B (MAOB) ) rabbit polyclonal antibody (STJ24454) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Monoamine Oxidase B (MAOB) rabbit polyclonal antibody (STJ24454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAOB antibody (400-490) (STJ24454)

SKU:
STJ24454

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAOB (400-490) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAOB
Gene ID: 4129
Uniprot ID: AOFB_HUMAN
Immunogen Region: 400-490
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-490 of human Monoamine Oxidase B (MAOB) (NP_000889.3).
Immunogen Sequence: TYFPPGILTQYGRVLRQPVD RIYFAGTETATHWSGYMEGA VEAGERAAREILHAMGKIPE DEIWQSEPESVDVPAQPITT TFLERHLPSVP
Function Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially degrades benzylamine and phenylethylamine.
Protein Name Amine Oxidase Flavin-Containing B
Monoamine Oxidase Type B
Mao-B
Database Links Reactome: R-HSA-141333
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Amine Oxidase Flavin-Containing B antibody
Anti-Monoamine Oxidase Type B antibody
Anti-Mao-B antibody
Anti-MAOB antibody

Information sourced from Uniprot.org

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