• Western blot analysis of extracts of various cell lines, using ARMET/ARP/MANF antibody (STJ29085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using ARMET/ARP/MANF antibody (STJ29085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using ARMET/ARP/MANF rabbit polyclonal antibody (STJ29085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g ARMET/ARP/MANF antibody (STJ29085). Western blot was performed from the immunoprecipitate using ARMET/ARP/MANF antibody (STJ29085) at a dilution of 1:1000.

Anti-MANF antibody (25-182) (STJ29085)

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STJ29085

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MANF (25-182) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MANF
Gene ID: 7873
Uniprot ID: MANF_HUMAN
Immunogen Region: 25-182
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-182 of human ARMET/ARP/MANF (NP_006001.5).
Immunogen Sequence: LRPGDCEVCISYLGRFYQDL KDRDVTFSPATIENELIKFC REARGKENRLCYYIGATDDA ATKIINEVSKPLAHHIPVEK ICEKLKKKDSQICELKYDKQ IDLSTVDLKKLRVKELKKIL DDWGETCKGCAEKSDYIRKI NELMPKYAPKAASARTDL
Post Translational Modifications May contain sialic acid residues.
Function Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.
Protein Name Mesencephalic Astrocyte-Derived Neurotrophic Factor
Arginine-Rich Protein
Protein Armet
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Retained In The Endoplasmic Reticulum (Er)
And Sarcoplasmic Reticulum (Sr) Under Normal Conditions
Up-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia
Alternative Antibody Names Anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor antibody
Anti-Arginine-Rich Protein antibody
Anti-Protein Armet antibody
Anti-MANF antibody
Anti-ARMET antibody
Anti-ARP antibody

Information sourced from Uniprot.org

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