• Immunohistochemistry analysis of MAN1A2 in paraffin-embedded Human colon carcinoma using MAN1A2 Rabbit polyclonal antibody (STJ116484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MAN1A2 Rabbit polyclonal antibody (STJ116484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAN1A2 antibody (60-180) (STJ116484)

SKU:
STJ116484

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAN1A2 (60-180) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAN1A2
Gene ID: 10905
Uniprot ID: MA1A2_HUMAN
Immunogen Region: 60-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human MAN1A2 (NP_006690.1).
Immunogen Sequence: SSKHKRFDLGLEDVLIPHVD AGKGAKNPGVFLIHGPDEHR HREEEERLRNKIRADHEKAL EEAKEKLRKSREEIRAEIQT EKNKVVQEMKIKENKPLPPV PIPNLVGIRGGDPEDNDIRE K
Tissue Specificity Highest levels of expression in placenta and testis.
Function Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
Protein Name Mannosyl-Oligosaccharide 1 -2-Alpha-Mannosidase Ib
Mannosidase Alpha Class 1a Member 2
Processing Alpha-1 -2-Mannosidase Ib
Alpha-1 -2-Mannosidase Ib
Database Links Reactome: R-HSA-6811438
Reactome: R-HSA-964827
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Mannosyl-Oligosaccharide 1 -2-Alpha-Mannosidase Ib antibody
Anti-Mannosidase Alpha Class 1a Member 2 antibody
Anti-Processing Alpha-1 -2-Mannosidase Ib antibody
Anti-Alpha-1 -2-Mannosidase Ib antibody
Anti-MAN1A2 antibody
Anti-MAN1B antibody

Information sourced from Uniprot.org

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