Anti-MAML1/Mastermind antibody (N-Term) (STJ70419)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70419-100
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General Information

Short DescriptionGoat polyclonal antibody anti-MAML1/Mastermind (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
ApplicationsPep-ELISA/WB/IHC
HostGoat
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 110kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108kDa according to NP_055572.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolMAML1
Gene ID9794
Uniprot IDMAML1_HUMAN
Accession NumberNP_055572.1
Immunogen RegionN-Term
Immunogen SequenceVLPTCPMAEFALPR

Additional Info

Function Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Protein Name Mastermind-Like Protein 1
Mam-1
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-8941856
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9793380
Cellular Localisation Nucleus Speckle
Nuclear
In A Punctate Manner
Alternative Antibody Names Anti-Mastermind-Like Protein 1 antibody
Anti-Mam-1 antibody
Anti-MAML1 antibody
Anti-KIAA0200 antibody

Information sourced from Uniprot.org

Citations

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