• Immunohistochemistry analysis of MAK in paraffin-embedded human placenta using MAK Rabbit polyclonal antibody (STJ119600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAK in paraffin-embedded human colon carcinoma using MAK Rabbit polyclonal antibody (STJ119600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAK in paraffin-embedded human liver using MAK Rabbit polyclonal antibody (STJ119600) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MAK Rabbit polyclonal antibody (STJ119600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAK antibody (370-435) (STJ119600)

SKU:
STJ119600

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAK (370-435) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAK
Gene ID: 4117
Uniprot ID: MAK_HUMAN
Immunogen Region: 370-435
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 370-435 of human MAK (NP_005897.1).
Immunogen Sequence: TLFPSIVKNMPTKPNGTLSH KSGRRRWGQTIFKSGDSWEE LEDYDFGASHSKKPSMGVFK EKRKKD
Tissue Specificity Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
Post Translational Modifications Autophosphorylated. Phosphorylated on serine and threonine residues.
Function Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Protein Name Serine/Threonine-Protein Kinase Mak
Male Germ Cell-Associated Kinase
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Midbody
Cell Projection
Cilium
Photoreceptor Outer Segment
Photoreceptor Inner Segment
Localized In Both The Connecting Cilia And The Outer Segment Axonemes
Localized Uniformly In Nuclei During Interphase
To The Mitotic Spindle And Centrosomes During Metaphase And Anaphase
And Also To Midbody At Anaphase Until Telophase
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Mak antibody
Anti-Male Germ Cell-Associated Kinase antibody
Anti-MAK antibody

Information sourced from Uniprot.org

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