Anti-MAFB antibody (184-323) (STJ112117)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ112117
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General Information

Short DescriptionRabbit polyclonal MAFB (184-323) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAFB
Gene ID9935
Uniprot IDMAFB_HUMAN
Immunogen Region184-323
Immunogen SequencePTSHPGPGPHATASATAAGG NGSVEDRFSDDQLVSMSVRE LNRHLRGFTKDEVIRLKQKR RTLKNRGYAQSCRYKRVQQK HHLENEKTQLIQQVEQLKQE VSRLARERDAYKVKCEKLAN SGFREAGSTSDSPSSPEFFL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 184-323 of human MAFB (NP_005452.2).

Additional Info

Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated by GSK3 and MAPK13 on serine and threonine residues. Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
Function Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain.
Protein Name Transcription Factor Mafb
Maf-B
V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B
Database Links Reactome: R-HSA-5617472
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Mafb antibody
Anti-Maf-B antibody
Anti-V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B antibody
Anti-MAFB antibody
Anti-KRML antibody

Information sourced from Uniprot.org

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