• Western blot analysis of the lysates from HepG2 cells using MADD antibody.
  • Western blot analysis of lysates from COLO, HeLa, and Jurkat cells, using MADD Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MADD Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using MADD Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using MADD Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using MADD Polyclonal Antibody diluted at 1：1000

Anti-MADD antibody (751-800 aa) (STJ93985)

SKU:
STJ93985

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP kinase-activating death domain protein (751-800 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MADD
Gene ID: 8567
Uniprot ID: MADD_HUMAN
Immunogen Region: 751-800 aa
Specificity: MADD Polyclonal Antibody detects endogenous levels of MADD protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MADD at the amino acid range 751-800
Function Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family. Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion. Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses. Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals. Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2. May link TNFRSF1A with MAP kinase activation. May be involved in the regulation of TNFA-induced apoptosis.
Protein Name Map Kinase-Activating Death Domain Protein
Differentially Expressed In Normal And Neoplastic Cells
Insulinoma Glucagonoma Clone 20
Rab3 Gdp/Gtp Exchange Factor
Rabgef
Rab3 Gdp/Gtp Exchange Protein
Rab3gep
Database Links Reactome: R-HSA-5357905
Reactome: R-HSA-8876198 Q8WXG6-3
Cellular Localisation Cell Membrane
Cytoplasm
Cell Projection
Axon
Alternative Antibody Names Anti-Map Kinase-Activating Death Domain Protein antibody
Anti-Differentially Expressed In Normal And Neoplastic Cells antibody
Anti-Insulinoma Glucagonoma Clone 20 antibody
Anti-Rab3 Gdp/Gtp Exchange Factor antibody
Anti-Rabgef antibody
Anti-Rab3 Gdp/Gtp Exchange Protein antibody
Anti-Rab3gep antibody
Anti-MADD antibody
Anti-DENN antibody
Anti-IG20 antibody
Anti-KIAA0358 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance