Anti-MAD2L1 antibody (70-150) [S6MR] (STJ11101336)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Mad2L1 (70-150) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S6MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:100-1:800ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAD2L1 |
| Gene ID | 4085 |
| Uniprot ID | MD2L1_HUMAN |
| Immunogen Region | 70-150 |
| Immunogen Sequence | EQLKDWLYKCSVQKLVVVIS NIESGEVLERWQFDIECDKT AKDDSAPREKSQKAIQDEIR SVIRQITATVTFLPLLEVSC S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 70-150 of human MAD2/MAD2L1 (Q13257). |
Additional Info
| Post Translational Modifications | Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2. |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. |
| Protein Name | Mitotic Spindle Assembly Checkpoint Protein Mad2aHsmad2Mitotic Arrest Deficient 2-Like Protein 1Mad2-Like Protein 1 |
| Database Links | Reactome: R-HSA-141405Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindle PoleRecruited By Mad1l1 To Unattached Kinetochores (Probable)Recruited To The Nuclear Pore Complex By Tpr During InterphaseRecruited To Kinetochores In Late Prometaphase After Bub1CenpfBub1b And CenpeKinetochore Association Requires The Presence Of Nek2Kinetochore Association Is Repressed By UbdSequestered To The Cytoplasm Upon Interaction With Isoform 3 Of Mad1l1 |
| Alternative Antibody Names | Anti-Mitotic Spindle Assembly Checkpoint Protein Mad2a antibodyAnti-Hsmad2 antibodyAnti-Mitotic Arrest Deficient 2-Like Protein 1 antibodyAnti-Mad2-Like Protein 1 antibodyAnti-MAD2L1 antibodyAnti-MAD2 antibody |
Information sourced from Uniprot.org