Anti-Macrophages antibody [25F9] (STJ16100303)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
STJ16100303-1
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General Information

Short DescriptionMouse monoclonal anti-Macrophages for use in IHC-F, FC, IHC-P and WB in Human and Rhesus samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-F/FC/IHC-P/WB
HostMouse
ReactivityHuman/Rhesus
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID25F9
IsotypeIgG1
ConjugationUnconjugated
Concentration100 Mu g/mL
Dilution RangeFC 1:10
WB 1:10
IHC 1:10
FormulationPBS with 0.1% BSA and 0.02% sodium azide
Storage InstructionStore at 2-8°C for up to 1-year, upon receipt.

Target Information

Additional Info

Background The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.

Information sourced from Uniprot.org

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