Anti-MACROH2A1 antibody (STJA0009422)

SKU:
STJA0009422

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Core histone macro-H2A.1 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MACROH2A1
Gene ID: 9555
Uniprot ID: H2AY_HUMAN
Specificity: This antibody detects endogenous levels of H2AFY at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human H2AFY
Post Translational Modifications Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. Isoform 1: Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism. Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them. This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration. Increases the expression of genes involved in redox metabolism, including SOD3. Isoform 2: In contrast to isoform 1, does not bind poly-ADP-ribose. Represses SOD3 gene expression.
Protein Name Core Histone Macro-H2a.1
Histone Macroh2a1
Mh2a1
Histone H2a.y
H2a/Y
Medulloblastoma Antigen Mu-Mb-50.205
Cellular Localisation Nucleus
Chromosome
Enriched In Inactive X Chromosome Chromatin And In Senescence-Associated Heterochromatin
Recruited To Dna Damage Sites In An Aplf-Dependent Manner
Alternative Antibody Names Anti-Core Histone Macro-H2a.1 antibody
Anti-Histone Macroh2a1 antibody
Anti-Mh2a1 antibody
Anti-Histone H2a.y antibody
Anti-H2a/Y antibody
Anti-Medulloblastoma Antigen Mu-Mb-50.205 antibody
Anti-MACROH2A1 antibody
Anti-H2AFY antibody

Information sourced from Uniprot.org

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