Anti-MACF1 antibody (5623-5673 aa) (STJ197593)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human MACF1 at the amino acid range 5623-5673
General Information
| Short Description | Rabbit polyclonal anti-Microtubule-actin cross-linking factor 1, isoforms 1/2/3/4/5 (5623-5673 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MACF1 |
| Gene ID | 23499 |
| Uniprot ID | MACF1_HUMAN |
| Immunogen | Synthesized peptide derived from the human MACF1 at the amino acid range 5623-5673 |
| Immunogen Region | 5623-5673 aa |
| Specificity | This antibody detects endogenous levels of human MACF1 |
Additional Info
| Post Translational Modifications | Phosphorylated on serine residues in the C-terminal tail by GSK3B. Phosphorylation inhibits microtubule-binding and this plays a critical role in bulge stem cell migration and skin wound repair. Wnt-signaling can repress phosphorylation. |
| Function | Isoform 2: F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells. As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development. |
| Protein Name | Microtubule-Actin Cross-Linking Factor 1 - Isoforms 1/2/3/4/5620 Kda Actin-Binding ProteinAbp620Actin Cross-Linking Family Protein 7Macrophin-1Trabeculin-Alpha |
| Database Links | |
| Cellular Localisation | Isoform 2: CytoplasmCytoskeletonCytoplasmGolgi ApparatusCell MembraneCell ProjectionRuffle MembraneThe Phosphorylated Form Is Found In The Cytoplasm While The Non-Phosphorylated Form Associates With The MicrotubulesLocalizes To The Tips Of MicrotubulesAssociated With The Minus-End Of Microtubules Via Interaction With Camsap3Apc Controls Its Localization To The Cell Membrane Which Is Critical For Its Function In Microtubule StabilizationIsoform 1: Cytoplasm |
| Alternative Antibody Names | Anti-Microtubule-Actin Cross-Linking Factor 1 - Isoforms 1/2/3/4/5 antibodyAnti-620 Kda Actin-Binding Protein antibodyAnti-Abp620 antibodyAnti-Actin Cross-Linking Family Protein 7 antibodyAnti-Macrophin-1 antibodyAnti-Trabeculin-Alpha antibodyAnti-MACF1 antibodyAnti-ABP620 antibodyAnti-ACF7 antibodyAnti-KIAA0465 antibodyAnti-KIAA0754 antibodyAnti-KIAA1251 antibody |
Information sourced from Uniprot.org