Anti-lysyl oxidase antibody (Internal) (STJ73225)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-lysyl oxidase (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 30-33kDa band observed in Human Heart and Human Placenta lysates (calculated MW of 46.9kDa according to NP_002308.2). The observed molecular weight corresponds to earlier findi |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | LOX |
| Gene ID | 4015 |
| Uniprot ID | LYOX_HUMAN |
| Accession Number | NP_002308.2; NP_001171573.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | STAYRADVRDYDHR |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_002308.2; NP_001171573.1). |
Additional Info
| Post Translational Modifications | The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin. Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity. Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen. |
| Function | Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture. |
| Protein Name | Protein-Lysine 6-OxidaseLysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form |
| Database Links | Reactome: R-HSA-1566948Reactome: R-HSA-2243919 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Protein-Lysine 6-Oxidase antibodyAnti-Lysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form antibodyAnti-LOX antibody |
Information sourced from Uniprot.org