• Western blot analysis of the lysates from HeLa cells using LYPLA1 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COLO cells, using LYPLA1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using LYPLA1 Polyclonal Antibody diluted at 1：2000

Anti-LYPLA1 antibody (51-100 aa) (STJ93974)

SKU:
STJ93974

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acyl-protein thioesterase 1 (51-100 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYPLA1
Gene ID: 10434
Uniprot ID: LYPA1_HUMAN
Immunogen Region: 51-100 aa
Specificity: LYPLA1 Polyclonal Antibody detects endogenous levels of LYPLA1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human LYPLA1 at the amino acid range 51-100
Function Acts as a acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Could also depalmitoylate ADRB2. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
Protein Name Acyl-Protein Thioesterase 1
Apt-1
Hapt1
Lysophospholipase 1
Lysophospholipase I
Lpl-I
Lysopla I
Palmitoyl-Protein Hydrolase
Database Links Reactome: R-HSA-203615
Reactome: R-HSA-9648002
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus Membrane
Endoplasmic Reticulum
Shows Predominantly A Cytoplasmic Localization With A Weak Expression In The Cell Membrane
Nuclear Membrane And Endoplasmic Reticulum
Alternative Antibody Names Anti-Acyl-Protein Thioesterase 1 antibody
Anti-Apt-1 antibody
Anti-Hapt1 antibody
Anti-Lysophospholipase 1 antibody
Anti-Lysophospholipase I antibody
Anti-Lpl-I antibody
Anti-Lysopla I antibody
Anti-Palmitoyl-Protein Hydrolase antibody
Anti-LYPLA1 antibody
Anti-APT1 antibody
Anti-LPL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance