Host: | Mouse |
Applications: | FC/WB/IHC/IP |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Mouse monoclonal antibody anti-Lymphocytic Marker is suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
Clonality : | Monoclonal |
Clone ID : | IPO-74 |
Conjugation: | Unconjugated |
Isotype: | IgG1k |
Formulation: | PBS with 0.02% Sodium Azide. |
Purification: | Affinity purified from tissue culture. |
Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
Dilution Range: | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (1, 0-2, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH6, 0, for 10-20 min f |
Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
Immunogen: | A BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653. |
Background | IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones. |
Information sourced from Uniprot.org