• STJ70604 (1µg/ml) staining of Human Liver (A) and Kidney (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70604 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70604 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-LXR alpha/LXR beta antibody (Internal) (STJ70604)

SKU:
STJ70604-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LXR alpha/LXR beta (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei of A549 cells. 10µg/ml
FC-Flow cytometric analysis of A549 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NR1H3
Gene ID: 10062
Uniprot ID: NR1H3_HUMAN
Immunogen Region: Internal
Accession Number: NP_005684.2; NP_009052.4;NP_001123573.1; NP_001238863.1; NP_001350524.1
Specificity: This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
Immunogen Sequence: CRLQDKKLPPLLSEI
Post Translational Modifications Ubiquitinated leading to its degradation by the proteasome.
Function Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators.
Protein Name Oxysterols Receptor Lxr-Alpha
Liver X Receptor Alpha
Nuclear Receptor Subfamily 1 Group H Member 3
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-383280
Reactome: R-HSA-4090294 Q13133-1
Reactome: R-HSA-8866427
Reactome: R-HSA-9029558
Reactome: R-HSA-9029569
Reactome: R-HSA-9031525
Reactome: R-HSA-9031528
Reactome: R-HSA-9623433
Reactome: R-HSA-9632974
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Oxysterols Receptor Lxr-Alpha antibody
Anti-Liver X Receptor Alpha antibody
Anti-Nuclear Receptor Subfamily 1 Group H Member 3 antibody
Anti-NR1H3 antibody
Anti-LXRA antibody

Information sourced from Uniprot.org

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