| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LRP2 (4446-4655) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:1000IHC-P 1:20-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LRP2 |
| Gene ID: |
4036 |
| Uniprot ID: |
LRP2_HUMAN |
| Immunogen Region: |
4446-4655 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human LRP2 (NP_004516.2). |
| Immunogen Sequence: |
FHYRRTGSLLPALPKLPSLS SLVKPSENGNGVTFRSGADL NMDIGVSGFGPETAIDRSMA MSEDFVMEMGKQPIIFENPM YSARDSAVKVVQPIQVTVSE NVDNKNYGSPINPSEIVPET NPTSPAADGTQVTKWNLFKR KSKQTTNFENPIYAQMENEQ KESVAATPPPSPSLPAKPKP PSRRDPTPTYSATEDTFKDT ANLVKEDSEV |
| Tissue Specificity | Expressed in first and third trimester cytotrophoblasts in the placenta (at protein level). Absorptive epithelia, including renal proximal tubules. |
| Post Translational Modifications | A fraction undergoes proteolytic cleavage of the extracellular domain at the cell membrane to generate a cytoplasmic tail fragment. This is internalized into the early endosome from where it trafficks in an LDLRAP1/ARH-dependent manner to the endocytic recycling compartment (ERC). In the ERC, it is further cleaved by gamma-secretase to release a fragment which translocates to the nucleus and mediates transcriptional repression. N-glycosylation is required for ligand binding. |
| Function | Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensis 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear. |
| Protein Name | Low-Density Lipoprotein Receptor-Related Protein 2Lrp-2Glycoprotein 330Gp330Megalin |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-975634Reactome: R-HSA-9758890 |
| Cellular Localisation | Apical Cell MembraneSingle-Pass Type I Membrane ProteinEndosome LumenMembraneCoated PitCell ProjectionDendriteAxonLocalizes To Brush Border Membranes In The KidneyIn The Endolymphatic Sac Of The Inner EarLocated In The Lumen Of Endosomes As A Soluble Form |
| Alternative Antibody Names | Anti-Low-Density Lipoprotein Receptor-Related Protein 2 antibodyAnti-Lrp-2 antibodyAnti-Glycoprotein 330 antibodyAnti-Gp330 antibodyAnti-Megalin antibodyAnti-LRP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
