Anti-LRAT antibody (1-194) (STJ115221)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LRAT (1-194) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LRAT |
| Gene ID | 9227 |
| Uniprot ID | LRAT_HUMAN |
| Immunogen Region | 1-194 |
| Immunogen Sequence | MKNPMLEVVSLLLEKLLLIS NFTLFSSGAAGEDKGRNSFY ETSSFHRGDVLEVPRTHLTH YGIYLGDNRVAHMMPDILLA LTDDMGRTQKVVSNKRLILG VIVKVASIRVDTVEDFAYGA NILVNHLDESLQKKALLNEE VARRAEKLLGFTPYSLLWNN CEHFVTYCRYGTPISPQSDK FCETVKIIIRDQRS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2). |
Additional Info
| Tissue Specificity | Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain. |
| Function | Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium.due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology. |
| Protein Name | Lecithin Retinol AcyltransferasePhosphatidylcholine--Retinol O-Acyltransferase |
| Database Links | Reactome: R-HSA-2453902Reactome: R-HSA-975634Reactome: R-HSA-9918442 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinRough Endoplasmic ReticulumEndosomeMultivesicular BodyCytoplasmPerinuclear RegionPresent In The Rough Endoplasmic Reticulum And Multivesicular Body In Hepatic Stellate CellsPresent In The Rough Endoplasmic Reticulum And Perinuclear Region In Endothelial Cells |
| Alternative Antibody Names | Anti-Lecithin Retinol Acyltransferase antibodyAnti-Phosphatidylcholine--Retinol O-Acyltransferase antibodyAnti-LRAT antibody |
Information sourced from Uniprot.org