• Immunofluorescence analysis of MCF7 cells using LRAT antibody (STJ115221). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Lrat antibody (STJ115221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-LRAT antibody (1-194) (STJ115221)

SKU:
STJ115221

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LRAT (1-194) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRAT
Gene ID: 9227
Uniprot ID: LRAT_HUMAN
Immunogen Region: 1-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2).
Immunogen Sequence: MKNPMLEVVSLLLEKLLLIS NFTLFSSGAAGEDKGRNSFY ETSSFHRGDVLEVPRTHLTH YGIYLGDNRVAHMMPDILLA LTDDMGRTQKVVSNKRLILG VIVKVASIRVDTVEDFAYGA NILVNHLDESLQKKALLNEE VARRAEKLLGFTPYSLLWNN CEHFVTYCRYGTPISPQSDK FCETVKIIIRDQRS
Tissue Specificity Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain.
Function Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium.due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology.
Protein Name Lecithin Retinol Acyltransferase
Phosphatidylcholine--Retinol O-Acyltransferase
Database Links Reactome: R-HSA-2453864
Reactome: R-HSA-2453902
Reactome: R-HSA-975634
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Rough Endoplasmic Reticulum
Endosome
Multivesicular Body
Cytoplasm
Perinuclear Region
Present In The Rough Endoplasmic Reticulum And Multivesicular Body In Hepatic Stellate Cells
Present In The Rough Endoplasmic Reticulum And Perinuclear Region In Endothelial Cells
Alternative Antibody Names Anti-Lecithin Retinol Acyltransferase antibody
Anti-Phosphatidylcholine--Retinol O-Acyltransferase antibody
Anti-LRAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance