• Immunofluorescence analysis of U2OS cells using Lamin B2 antibody (STJ28566). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human adenomyosis using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Lamin B2 antibody (STJ28566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LMNB2 antibody (341-620) (STJ28566)

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STJ28566

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LMNB2 (341-620) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB2
Gene ID: 84823
Uniprot ID: LMNB2_HUMAN
Immunogen Region: 341-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human Lamin B2 (NP_116126.3).
Immunogen Sequence: AMAGERDKFRKMLDAKEQEM TEMRDVMQQQLAEYQELLDV KLALDMEINAYRKLLEGEEE RLKLSPSPSSRVTVSRATSS SSGSLSATGRLGRSKRKRLE VEEPLGSGPSVLGTGTGGSG GFHLAQQASASGSVSIEEID LEGKFVQLKNNSDKDQSLGN WRIKRQVLEGEEIAYKFTPK YILRAGQMVTVWAAGAGVAH SPPSTLVWKGQSSWGTGESF RTVLVNADGEEVAMRTVKK
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B2
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B2 antibody
Anti-LMNB2 antibody
Anti-LMN2 antibody

Information sourced from Uniprot.org

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