• Immunofluorescence analysis of U-2 OS cells using LMAN1 Rabbit polyclonal antibody (STJ112470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using LMAN1 Rabbit polyclonal antibody (STJ112470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using LMAN1 Rabbit polyclonal antibody (STJ112470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using LMAN1 Rabbit polyclonal antibody (STJ112470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LMAN1 antibody (270-480) (STJ112470)

SKU:
STJ112470

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LMAN1 (270-480) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMAN1
Gene ID: 3998
Uniprot ID: LMAN1_HUMAN
Immunogen Region: 270-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1).
Immunogen Sequence: PGKEPPTPDKEISEKEKEKY QEEFEHFQQELDKKKEEFQK GHPDLQGQPAEEIFESVGDR ELRQVFEGQNRIHLEIKQLN RQLDMILDEQRRYVSSLTEE ISKRGAGMPGQHGQITQQEL DTVVKTQHEILRQVNEMKNS MSETVRLVSGMQHPGSAGGV YETTQHFIDIKEHLHIVKRD IDNLVQRNMPSNEKPKCPEL PPFPSCLSTVH
Tissue Specificity Ubiquitous.
Post Translational Modifications The N-terminal may be partly blocked.
Function Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Protein Name Protein Ergic-53
Er-Golgi Intermediate Compartment 53 Kda Protein
Gp58
Intracellular Mannose-Specific Lectin Mr60
Lectin Mannose-Binding 1
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-948021
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus Membrane
Single-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Protein Ergic-53 antibody
Anti-Er-Golgi Intermediate Compartment 53 Kda Protein antibody
Anti-Gp58 antibody
Anti-Intracellular Mannose-Specific Lectin Mr60 antibody
Anti-Lectin Mannose-Binding 1 antibody
Anti-LMAN1 antibody
Anti-ERGIC53 antibody
Anti-F5F8D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance