![Immunohistochemistry analysis of LITAF in paraffin-embedded human prostate using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101119/388443/STJ27422_1__91566.1713157944.jpg?c=1 "Immunohistochemistry analysis of LITAF in paraffin-embedded human prostate using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and LITAF knockout (KO) HeLa cells, using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101119/388445/STJ27422_3__21667.1713157946.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LITAF knockout (KO) HeLa cells, using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry analysis of LITAF in paraffin-embedded human prostate using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101119/388443/STJ27422_1__91566.1713157944.jpg?c=1 "Immunohistochemistry analysis of LITAF in paraffin-embedded human prostate using [KO Validated] LITAF Rabbit polyclonal antibody (STJ27422) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
Anti-LITAF antibody (1-161) (STJ27422)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LITAF (1-161) antibody for use in WB, IHC-P, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LITAF |
| Gene ID | 9516 |
| Uniprot ID | LITAF_HUMAN |
| Immunogen Region | 1-161 |
| Immunogen Sequence | MSVPGPYQAATGPSSAPSAP PSYEETVAVNSYYPTPPAPM PGPTTGLVTGPDGKGMNPPS YYTQPAPIPNNNPITVQTVY VQHPITFLDRPIQMCCPSCN KMIVSQLSYNAGALTWLSCG SLCLLGCIAGCCFIPFCVDA LQDVDHYCPNCRALLGTYKR L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LITAF (NP_004853.2). |
Additional Info
| Tissue Specificity | Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. |
| Post Translational Modifications | Phosphorylated on tyrosine residues in response to EGF. |
| Function | Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps down-regulate downstream signaling cascades. Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines. May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10. |
| Protein Name | Lipopolysaccharide-Induced Tumor Necrosis Factor-Alpha FactorLps-Induced Tnf-Alpha FactorSmall Integral Membrane Protein Of Lysosome/Late EndosomeP53-Induced Gene 7 Protein |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusLysosome MembranePeripheral Membrane ProteinCytoplasmic SideEarly Endosome MembraneLate Endosome MembraneEndosome MembraneCell MembraneGolgi Apparatus MembraneAssociated With Membranes Of LysosomesEarly And Late EndosomesCan Translocate From The Cytoplasm Into The NucleusDetected At Schmidt-Lanterman Incisures And In Nodal Regions Of Myelinating Schwann Cells |
| Alternative Antibody Names | Anti-Lipopolysaccharide-Induced Tumor Necrosis Factor-Alpha Factor antibodyAnti-Lps-Induced Tnf-Alpha Factor antibodyAnti-Small Integral Membrane Protein Of Lysosome/Late Endosome antibodyAnti-P53-Induced Gene 7 Protein antibodyAnti-LITAF antibodyAnti-PIG7 antibodyAnti-SIMPLE antibody |
Information sourced from Uniprot.org