Anti-Lipocalin-2/NGAL antibody (21-200) [S5292RM] (STJ11105292)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Lipocalin-2/NGAL (21-200) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5292RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Lcn2 |
| Gene ID | 16819 |
| Uniprot ID | NGAL_MOUSE |
| Immunogen Region | 21-200 |
| Immunogen Sequence | QDSTQNLIPAPSLLTVPLQP DFRSDQFRGRWYVVGLAGNA VQKKTEGSFTMYSTIYELQE NNSYNVTSILVRDQDQGCRY WIRTFVPSSRAGQFTLGNMH RYPQVQSYNVQVATTDYNQF AMVFFRKTSENKQYFKITLY GRTKELSPELKERFTRFAKS LGLKDDNIIFSVPTDQCIDN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 21-200 of mouse Lipocalin-2/NGAL (NP_032517.1). |
Additional Info
| Tissue Specificity | Expressed in the cortical tubules of the kidney (at protein level). Also expressed in the medullary tubules of the kidney. Detected in lung, spleen, uterus, vagina and epididymis. |
| Post Translational Modifications | N-glycosylated. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgalLipocalin-2Oncogene 24p324p3Sv-40-Induced 24p3 ProteinSiderocalin Lcn2P25 |
| Database Links | Reactome: R-MMU-6798695Reactome: -MMU-6799990Reactome: -MMU-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Names | Anti-Neutrophil Gelatinase-Associated Lipocalin antibodyAnti-Ngal antibodyAnti-Lipocalin-2 antibodyAnti-Oncogene 24p3 antibodyAnti-24p3 antibodyAnti-Sv-40-Induced 24p3 Protein antibodyAnti-Siderocalin Lcn2 antibodyAnti-P25 antibodyAnti-Lcn2 antibody |
Information sourced from Uniprot.org