Anti-Lime1-Mouse antibody (C-Term) (STJ70557)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Lime1-Mouse (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Accession Number | NP_076173.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | ENVYESIKEMGL |
Additional Info
| Post Translational Modifications | Palmitoylation of Cys-28 and Cys-31 is required for raft targeting. Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation.which leads to the recruitment of SH2-containing proteins. |
| Function | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. |
| Protein Name | Lck-Interacting Transmembrane Adapter 1Lck-Interacting Molecule |
| Cellular Localisation | Cell MembraneSingle-Pass Type Iii Membrane ProteinPresent In Lipid RaftsRecruited To The Immunological Synapse Upon Conjugation Of T-Cell With Antigen-Presenting Cell |
| Alternative Antibody Names | Anti-Lck-Interacting Transmembrane Adapter 1 antibodyAnti-Lck-Interacting Molecule antibodyAnti-Lime1 antibodyAnti-Lime antibody |
Information sourced from Uniprot.org