| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LIMA1 (560-759) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LIMA1 |
| Gene ID: |
51474 |
| Uniprot ID: |
LIMA1_HUMAN |
| Immunogen Region: |
560-759 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 560-759 of human LIMA1 (NP_057441.1). |
| Immunogen Sequence: |
DEISKPEVPEDVDLDLKKLR RSSSLKERSRPFTVAASFQS TSVKSPKTVSPPIRKGWSMS EQSEESVGGRVAERKQVENA KASKKNGNVGKTTWQNKESK GETGKRSKEGHSLEMENENL VENGADSDEDDNSFLKQQSP QEPKSLNWSSFVDNTFAEEF TTQNQKSQDVELWEGEVVKE LSVEEQIKRNRYYDEDEDEE |
| Tissue Specificity | Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium. |
| Post Translational Modifications | Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility. |
| Function | Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake. |
| Protein Name | Lim Domain And Actin-Binding Protein 1Epithelial Protein Lost In Neoplasm |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionCytoskeletonStress FiberCell MembraneExpressed In The Brush Border Membrane Of The Small Intestine And Colocalizes With Npc1l1 And Myo5bColocalizes With Pxn At Focal Adhesions In Mesangial CellsColocalizes With Actin Stress Fibers In Quiescent CellsPdgf Stimulation Induced Disassembly Of Stress Fibers And Formation Of Peripheral And Dorsal RufflesWhere Lima1 Is Relocalized |
| Alternative Antibody Names | Anti-Lim Domain And Actin-Binding Protein 1 antibodyAnti-Epithelial Protein Lost In Neoplasm antibodyAnti-LIMA1 antibodyAnti-EPLIN antibodyAnti-SREBP3 antibodyAnti-PP624 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
