• Western blot analysis of extracts of various cell lines, using LIM2 antibody (STJ110218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using LIM2 antibody (STJ110218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LIM2 antibody (25-110) (STJ110218)

SKU:
STJ110218

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIM2 (25-110) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIM2
Gene ID: 3982
Uniprot ID: LMIP_HUMAN
Immunogen Region: 25-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human LIM2 (NP_085915.2).
Immunogen Sequence: TDHWMQYRLSGSFAHQGLWR YCLGNKCYLQTDSIGEPPGQ GPGRAWGKSRADLGAQGHLY SRWRTLRLKEGKGATQAYWN ATRAFM
Tissue Specificity Eye lens specific.
Function Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.
Protein Name Lens Fiber Membrane Intrinsic Protein
Mp18
Mp19
Mp20
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Lens Fiber Membrane Intrinsic Protein antibody
Anti-Mp18 antibody
Anti-Mp19 antibody
Anti-Mp20 antibody
Anti-LIM2 antibody

Information sourced from Uniprot.org

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